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Tip of the
Month:
DNA
Profiling
Deoxyribonucleic acid(DNA) is
a specialized molecule that encodes all of the
genetic information of an individual. A child receives one half
of his or
her DNA from each biological parent, forming a pattern, or "profile" that
is
unique from individual to individual, with the exception of identical
twins.
By comparing the DNA characteristics of the mother and child
it is possible
to identify the characteristics that the child inherited from
the biological
mother. Thus, any remaining DNA must have come from the biological
father.
If the DNA profile from the tested man is found to contain these
obligatory
paternal characteristics, then a statistical analysis can be
performed to
determine the probability of paternity.
At Fairfax Identity Laboratories, a tested man will either be
excluded by 2 or more genetic locations or included with a probability
of paternity
greater than 99.0%. for PCR testing and greater than 99.9% for
RFLP testing.
Specimen Requirements
Specimens must be collected under strict chain of custody protocol.
Please call the laboratory for instructions.
Amniotic Fluid
At least 20 ml unspun amniotic fluid in red top centrifuge tube(s)
OR 2 T25 flasks of cultured amniocytes, 100% confluent.
Blood
Infant: 0.5ml venous or cord blood in EDTA(lavender top) tube.
Child(over 6 months):0.5-3 ml peripheral blood in EDTA(lavender
top) tube
Adult: 0.5ml-3ml peripheral blood in EDTA(lavender top) tube.
Buccal Swabs
2-4 swabs
CVS
10 mg cleaned villi in sterile medium in sterile centrifuge tube
OR 2 T25
flasks of cultured cells, 100% confluent.
Products of Conception, Tissue
10mg tissue in sterile medium. Seal tightly.
Testing Method
Multiple single-locus DNA analysis.
Turnaround Time
An analysis cannot be made until all samples involved in the
case have been
processed in the laboratory. Specimens are processed upon receipt
at our
facilities, and results are routinely available in about 9-10
business days
for normal Paternity. Call 1-800-482-3025 for other turnaround
times.
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